从中国对虾分离纯化的一种杆状病毒及其超微结构的研究

石拓; 孔杰; 包振民; 梁兴明; 刘萍; 王崇明

从中国对虾分离纯化的一种杆状病毒及其超微结构的研究

1.
中国水产科学研究院黄海水产研究所, 青岛266071
2.
青岛海洋大学海洋生命学院, 青岛266003

基金项目: 国家攀登计划B资助课题(编号:PDB6-6-1)

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出版历程
- 收稿日期: 1996-05-13
- 修回日期: 1997-03-06

Studies on Purification and ultrastructure of a baculoviral pathogen in Penaeus chinesis

1.
Yellow Sea Fisheries Research Insititute, Qingdao 266071
2.
College of Marine Life Sciences, Ocean University of Qingdao, Qingdao, 266003

摘要

摘要: 从患病和濒死的中国对虾(Penaeus chinensis)头胸部分离提纯出一种杆状病毒,同时经对病虾的鳃、胃和中肠组织进行超薄切片及电镜观察,亦在上述组织的细胞核内发现大小相同、形态一致的大量杆状病毒粒子.成簇的病毒粒子主要在核内装配,并导致细胞结构的病变.病毒粒子大小约为320～400nm×100～130nm,两端钝圆,中部膨大,有囊膜,分内外两后,核衣壳大小为250～300nm×70～100nm.细胞内未观察到有核型多角体或颗粒体类包涵体存在.根据病毒学分类原则,该病毒应属于杆状病毒属无包涵体杆状病毒亚群,即杆状病毒C亚群.
- 中国对虾 /
- 非包涵体杆状病毒 /
- 分离提纯 /
- 电镜观察
Abstract: A kind of baculovirus was islated from the mid-gut gland tissues of sick of morbid Penaeus chinensis.Electron microscopic examination of ultrathin section of the gills, hepatopancrease and mid-gut gland tissues also revealed the presence of rod-shaped baculovirus particles with same sine in the affected cell nuclei.The virion covered with bilaminal envelope was 320-400 nm×100-130 nm in size,whereas the nucleocapsid ranged in the size of 250-300 nm in length and 70-100 nm in breadth respectively.No occlusion bodies have been found in cells.According to the principle of viral classification, this new found virus can probably belong to the non-occluded subgroup of insect baculoviridae,i.e.,C subgroup baculovirus.
- Penaeus chinesis /
- non-occluded baculovirus /
- isolation and purification /
- electronic observation

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参考文献(17)

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