有机污染提高对虾对病原菌易感性研究
Organic pollution enhance susceptibilites of Penaeus chinensis to pathogenic bacteria
-
摘要: 1995年5～11月,以人工合成饵料作为有机污染源,取体长8、10和12cm以上中国对虾,在0.9m3水族箱中,分批进行有机污染提高对虾对病原体易感性试验.对虾在上述环境中培育2～3周后,测定其对付溶血性孤菌易感性及体内与抗病有关一些酶的活性.结果:与对照组相比,试验组对虾对病原菌易感性提高1～3倍;体内SOD、PO及溶菌酶活性分别下降约22%、50%和28%～86%.血细胞数量减少50%左右.这与人工合成饵料在水环境中经分解后,产生一些对对虾有害物质,其中以氨氮产生和DO减少尤为显着有关.Abstract: From May to November of 1995,P.chinensis were used experiments using excessive artifical diets as organic pollutants.Twenty about 8, 10 or 12cm body length P.chinensis were put into the test pond and control pond.After 2~3 weeks,susceptibilities to pathogenic bacteria the number of haemocyte and lysozyme,SOD, PO activities were compared.The results were as follows.Susceptibilities to Vitrio parahaemolyticus, were 1~3 times that of the control pond shrimp (Table 1),the haemocyte number of the test shrimps decreased about 50%,the SOD, PO and lysozyme activities of the test pond decreased about 22%, 50% and 28%-86% compared with those of the control pond (Table 2).Additionally it has been proved that in the test pond following the excessive artifical diets degreded,the DO content decreased,NH3-N increased markedly.So the conclusion could be drawn:organic pollution affects aquatic environment,decreases immune level,enhances susceptibilities to pathogen bacteria.
-
Key words:
- Penaeus chinenses /
- organic pollution /
- anti-disease ability /
- immune level
-
王方国.对虾养殖水质与饵料的关系研究.东海海洋,1995,(2):9-14 董存有,张金荣.虾塘COD的变化及其对对虾生长和产量的影响.海洋科学,1993,(3):8-12 郑碧玉,袁勤生,李文杰.改良后的连苯三酚自氧化测定son活性方法.生物化学与生物物理进展,18(2) :163-163 丁美丽,林林,李光友.有机污染对虾体内外环境影响研究.海洋与湖沼,1997,28(1):7-11 Waiters C:R,Plumb J A.Environmental stress and bacterial infection in channel Catfish,Ictalurus punctatus punctatus Ratinesque.J.Fish.Biol.,1980,17,171-185 Wedemeyer U A,Uoodyear C P.Disease caused by environmental stressors.Disease of Marine Animals,D.Biologische Anstalt Helgoland,Hamsurg,1984,4(1):424-434 Chu F L,Hale k C.Relationship between pollution and susceptibility to infections disease in the eastern oyster Crassostrea virginica.Mar.Environ.Res.,1994,38,243-256 Soderhall K.Prophenoloxidase activating system and melanization-a recognition mechanism of arthropods A review.Dev.Comp.Immunol.,1982,6,601-611 宋宏红,谢允廷,宋延令.虾子的防御系统与抗病力的增强.养鱼世界(台),1995,(2):22-23 张尧,宋延令.甲壳类血球细胞的免疫功能及其交互作用.中国水产(台),1993,(483):7-14 Malins D C,Myers M S,Roubal W T.Organic free radicals associated with idiopathic liver lesions of English Sele(Parophry vetulus) from polluted marine environments.Environ.Science and Technology,1983,17,679-685 李永棋,丁美丽。海洋污染生物学.北京:海洋出版社,1991,305-309 Zhou Zhigang.The influence of selenium on the antioxidation in spirulina.J.Phycology,Supplemend to Volume,1995,31(3):17 李文杰.超氧化物歧化酶在治疗超氧阴离子自由基所引起的疾病及抗衰老上的应用.中国药物杂志,1989,24(7):397-401 -
点击查看大图
计量
- 文章访问数: 970
- HTML全文浏览量: 4
- PDF下载量: 997
- 被引次数: 0
下载:
