中国对虾三倍体的诱导研究——Ⅱ.细胞松弛素B处理
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摘要: 用细胞松驰素B(C.B)处理中国对虾(Penaeus chinensis)的受精卵,诱发三倍体,经培养已获得溞状幼体.实验所用C.B浓度为0.1~2.5mg/dm3.处理的起始时间是在受精后5~20min,处理的持续时间为10~40min.三倍体的诱导率达62.5%.C.B处理浓度越高,对极体排放的抑制力越强,但胚胎畸形率和非整倍体数量则增多.对虾三倍体的诱导成功,为对虾的多倍体育种提供了可能.
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